ZPA Regulatory Sequence (ZRS) Activity in the Limb is Eliminated by Loss of Hand2, Twist1, and Hoxd13 Binding Sites

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Identification of spontaneous mutations within the long-range limb-specific Sonic hedgehog enhancer (ZRS) that alter Sonic hedgehog expression in the chicken limb mutants oligozeugodactyly and silkie breed.

The evolutionarily conserved, non-coding ~800-base-pair (bp) zone of polarizing activity (ZPA) regulatory sequence (ZRS) controls Shh expression in the posterior limb. We report that the chicken mutant oligozeugodactyly (ozd), which lacks limb Shh expression, has a large deletion within the ZRS. Furthermore, the preaxial polydactylous, Silkie Breed chicken, which develops ectopic anterior limb ...

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ژورنال

عنوان ژورنال: The FASEB Journal

سال: 2021

ISSN: 0892-6638,1530-6860

DOI: 10.1096/fasebj.2021.35.s1.04470